The purpose of agarose electrophoresis is to separate DNA based on size, as well as to visualize it after amplification or DNA extraction (e.g. such as plasmid or genomic DNA extraction). DNA can then be extracted from the gel for further analysis and processing.

The purpose is to amplify DNA so that it can be visible for analysis or further processing.

The purpose is to extract and purify genomic DNA using the PureLink® Genomic DNA kits by Invitrogen™.

The purpose is to prepare a discontinuous polyacrylamide gel used as a support medium for separating proteins by electrophoresis. A prepared gel of a given acrylamide concentration or type separates proteins effectively within a characteristic range.

This is to separate proteins or polypeptides according to their sizes or molecular weights using an electrophoretic method. The technique is performed in polyacrylamide gels containing sodium dodecyl sulfate (SDS) which is used to linearize proteins and to negatively charge the proteins. As a result, negatively charged proteins will migrate towards the positive electrode and will be fractionated by approximate size during electrophoresis.

This is to retain or fix the proteins within the gel matrix and to make the protein bands visible for analysis. Once the gel run is complete, a separate dye such as bromophenol blue will bind to the protein in the polyacrylamide gel for the proteins to be visible during analysis. Excess dye is removed during the destaining step with a wash solution consisting of the fixative solution minus the dye through a diffusion process.

This is to transfer proteins from the gel to the membrane support typically nitrocellulose or PVDF. The balance of SDS and methanol in the transfer buffer, protein size and gel percentage will affect transfer efficiency.

This is to identify genetic activity of specific genes within a group of cells as a response to various influences either from internal or external effects.

This is to measure the concentration of nucleic acid samples using the Eppendorf spectrophotometer with 1 mm cuvette.

This is to measure the concentration of protein samples using the Eppendorf spectrophotometer with 1 mm cuvette.

This is to prepare polyacrylamide gels which are used to separate shorter nucleic acids (double stranded DNA of up to 1000 base pairs), based on the concentration used. Different concentrations of acrylamide are necessary to optimize resolution of nucleic acids with different lengths. Gels provide a simple, low-cost way to separate nucleic acids based on size for quantification and purification.

 

This is to pour and run a neutral polyacrylamide gel for nucleic acid samples. Gel electrophoresis is used to separate macromolecules such as DNA, RNA and proteins. DNA fragments are separated according to their size.

This is for the visualization of the DNA/RNa bands within the gel corresponding to base pairs with different molecular weights. The most common dye used to make DNA or RNA bands visible for gel electrophoresis is Ethidium bromide (EtBr) which fluoresces under UV light when intercalated into the major groove of DNA (or RNA).

This is to preserve tissue samples permanently in as life-like state as possible and to allow for the preparation of thin, stained sections for histological analysis.

This is to measure the growth of bacteria using a 96-well plate instead of the classic cuvette method at OD600. The protocol only requires 100 μL of media to obtain the growth curve.

To quantify the level of bacterial biofilm formation using a spectrophotometric method.