Day 1: Lecture and Workshop
Postgraduate students, faculty members, and RSU research collaborators participated in the Confocal Microscopy lecture and training held on December 8-9, 2015 at Lecture Room No. 441016, New Dental Hospital Building highlighting topics on Principles of Fluorescence, Confocal Microscopy, and Digital Cellular Imaging. The two-day event also included workshop demonstrating the fundamental steps on how to operate the Confocal Microscope at the Molecular and Cell Biology Laboratory.
Dr. Ali Al Hoshani, a Ph.D. in Pharmacological Science explained the Principles of Fluorescence during Day 1 Lecture on Confocal Microscopy. He described that fluorescence refers to the physical property of the object absorbing light at one wavelength and then re-emitting it at another wavelength.
He illustrated the Excitation and Emission Diagram as well as Simplified Jablonski Diagram showing the energy stages; Stage 1: absorption, Stage 2: excited-state lifetime, and Stage 3: Fluorescence emission.
Dr. Khaled Al Hosaini, an Assistant Professor of Molecular Pharmacology, presented a lecture on Confocal Microscopy in Biomedical Research. He discussed the basic key to confocal approach for the use of spatial filtering techniques to eliminate out of focus light or glare in specimens whose thickness exceeds the mediate plane of focus.
He also highlighted the two major types of confocal scanning, which is the Laser Scanning Confocal Microscope (LSCM) and the Spinning Disk Scanning Confocal Microscope (SDSCM).
Day 2: Lecture and Workshop
Dr. Sundus Bukhary, an Endodontic Postgraduate student, showcased her study during Day 2 Lecture on Confocal Microscopy. Her presentation titled “The Use of Confocal Laser Scanning Microscopy for the Study of Dentin Infection” has illustrated the different techniques e.g. Microbiological, Histological and Transmission Electron Microscopy that studies the presence of bacteria inside dentinal tubules.
Two-day Confocal microscope Workshop